Six test tubes were set up. Two different solutions were added to each test tube as shown in the table.
The temperature, pH and all concentrations were optimal.
Which three mixtures would lead to the presence of amino acids in the test tube?
test tube mixture of solutions
1 lipase and lipid
2 lipase and boiled protein
3 protease and lipase
4 boiled protease and protein
5 protease and boiled protein
6 protease and protein

Answer:

17) F

Test tube 1 – lipases would break lipids. No amino acids would be found.
Test tube 2 – lipases break down lipids, not proteins. No amino acids would be found.
Test tube 3 – Protease can break down lipases (remember, lipase is an enzyme and enzymes are proteins)
Test tube 4 – protease can break down proteins. However, in test tube 4, the protease is boiled and hence, it would denature. So it would not break down the protein. No amino acids found in test tube 4
Test tube 5 – protease can break down boiled protein into amino acids
Test tube 6 – protease can break down protein into amino acids
Tube 3, 5 and 6 will have amino acids.